The Imaging Facility's ultra-high content/ultra-plex imaging platform is a Miltenyi MACSima. This system can theoretically image an unlimited number of targets in one sample with the sequential addition, imaging and removal of labelled antibodies. The system is fully automated, uses samples prepared on standard microscope slides and does not require the use of proprietary antibodies.
Our system:
Miltenyi MACSima
Fully automated ultra-high content/ultra-plex imaging platform
Wide-field epifluorescence microscope
Four channel detection (blue, green, red, far-red)
2x, 20x/0.75, 20x/0.45 objectives
Two techniques signal removal: photobleaching of primary conjugated antibodies or antibody release by enzymatic reaction
Automate liquid handling system
Ultraprecise stage for loading sample carriers
Hardware and image-based autofocus
MACS iQ view analysis software for imaging processing (multiplex fluorescence visualisation, segmentation, interactive gating, data analyses, multiple plotting options, workflow editor, distance analyses)
