Super Resolution Imaging

Super Resolution Imaging

We have a Nikon system which combines two different methods of super resolution imaging: Structured Illumination Microscopy (SIM) and Stochastic Optical Reconstruction Microscopy (STORM).  SIM is compatible with many commonly used fluorescent dyes and fluorescent proteins, but 'only' offers twice the resolution achievable with standard fluorescence microscopy.  STORM requires the use of specific fluorophores and a special imaging buffer, but can achieve an order of magnitude enhancement in the resolution over standard fluorescence microscopy.  Both methods have their strengths and weaknesses, but having the two options available means we can apply the best approach to a specific problem, or combine the techniques for a complimentary approach.

Our System:

Nikon N-SIM/N-STORM

Structured Illumination Microscopy

  • 405, 440, 488, 561 & 635 nm excitation

  • 60x water immersion, 100x oil immersion

  • 2D-SIM, 3D-SIM, TIRF SIM

  • Theoretical lateral resolution in the range of 120-180 nm

Stochastic Optical Reconstruction Microscopy

  • N-STORM

  • dSTORM

  • PALM

  • Theoretical lateral resolution in the range of 20-50 nm