Creating KO models using CRISPR/Cas9
CRISPR/Cas9 technology has made eliminating gene expression in cells routine, making KO models for research. This is because CRISPR/Cas9 can create double stranded breaks (DSB) nearly anywhere in the genome. SgRNA can positioned in specific places within a gene to prevent the targeted gene from producing any protein (Figure 1). This document will discuss different approaches in making KO mode.