Life Sciences Research for Lifelong Health
Post-Doc

Postdoctoral Research Scientist

Epigenetics

We are looking for a proficient and highly motivated Postdoctoral Research Scientist to lead a research project to investigate the role of Polycomb in controlling transcriptional heterogeneity and lineage priming in human pluripotent stem cells. The successful candidate will join Peter Rugg-Gunn’s team within the Epigenetics Programme. The Epigenetics Programme has major strengths in applying low cell number epigenomic assays to a wide range of questions in developmental and ageing biology.
 
The successful candidate will work with already established EZH2-deficient cell lines, and will additionally use CRISPR technology to engineer new cell lines with further Polycomb gene deletions. The post holder will characterise the mutant cells using single cell approaches (scRNA-seq; Fluidigm RT-qPCR; CyTOF) and low cell number epigenetic assays (ChIP-seq; ATAC-seq) to define the level of epigenetic and transcriptional heterogeneity.
 
To functionally associate the EZH2-dependent transcriptional changes with altered lineage priming, the post holder will flow sort lineage-primed cell populations and subject the isolated populations to self-renewal and directed differentiation assays. The overall significance of this work will be to characterise the molecular basis for multi-lineage priming in human pluripotent stem cells.
 
The successful candidate will have demonstrated experience in molecular biology including the preparation of RNA-seq and ChIP-seq libraries, and an extensive knowledge of stem cell biology and epigenetic research. Previous experience with low cell number epigenetic and transcriptional techniques is highly desirable.

Funding for this post is available for three years in the first instance.


For more information on the work of the group, please visit: https://babraham.ac.uk/our-research/epigenetics/peter-rugg-gunn
 
Starting salary is £30,751 per annum to £34,166 per annum depending on experience.
 
Full details of the position can be found here.
 
On applying please quote reference PRG-PD-BB.
 
Closing date for completed applications is Tuesday 21st November 2017.
 
NO AGENCIES PLEASE
 
The Babraham Institute is a Disability Confident Employer and has a positive approach to employing disabled people.
 
The Babraham Institute receives strategic funding from the Biotechnology and Biological Sciences Research Council and is an Equal Opportunities Employer.

 


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Closing date
21/11/2017

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