HiSeq High Output Sequencing
The HiSeq 2500 and 1000 instruments in High Output mode maximize throughput and data yield. As many as 200-300 million sequence reads may be obtained from a single lane on the HiSeq, and eight lanes are analysed simultaneously. The number of sequence reads obtained from the HiSeq allows a high level of multiplexing, and up to 96 libraries may be indexed and pooled in a single lane. Researchers analysing samples on the HiSeq can select from 50- or 100-bp reads and choose between Single-Read or Paired End sequencing runs.
HiSeq Rapid Run Sequencing
The HiSeq 2500 in Rapid Run mode enables researchers to obtain results faster, from a limited number of samples without reducing the depth of sequencing.
The Rapid Run mode of the HiSeq 2500 allows one or two samples to be analysed on a two-lane flowcell and produces 150-180 million reads per lane.
The MiSeq personal sequencer provides maximum flexibility with wide range of read lengths and short turnover times.
This instrument enables reseachers to select read lengths from 50- to 600-bp (the latter with a 2x300-bp Paired End sequencing run). The MiSeq has fast run times - a 50-cycle run takes just over 6 hours, while a 600-cycle can be completed in 2.25 days – as well as quick turnover times, making this instrument well suited for validation studies.