Life Sciences Research for Lifelong Health

Publications david-oxley

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Structure of the O-specific polysaccharide from the lipopolysaccharide of Serratia marcescens O8.
Oxley D,Wilkinson SG

Structural studies have been carried out on the O-specific polysaccharide from the lipopolysaccharide of the reference strain (CDC 1604-55) for serogroup O8 of Serratia marcescens. The polymer has a branched, tetrasaccharide repeating unit of D-galactose(Gal),D-glucose(Glc), and 2-acetamido-2-deoxy-D-glucose(GlcNAc) with the following structure: (Formula: see text). The anomeric configuration assigned to the glucose residue differs from that (beta) previously proposed [Tarcsay, L., Wang, C. S., Li, S.-C. and Alaupovic, P. (1973) Biochemistry 12, 1948-1955]. The structure of the O8 polymer is identical with that of one of two polymers present in the cell envelope of a strain (CDC 1783-57) of S. marcescens O14.

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European journal of biochemistry / FEBS, 156, 0014-2956, 597-601, 1986

PMID: 2422032

Structure of a neutral glycan isolated from the lipopolysaccharide of the reference strain for Serratia marcescens serogroup O22.
Oxley D,Wilkinson SG

Both a neutral and an acidic polymer have been isolated from a lipopolysaccharide extract of the reference strain for Serratia marcescens serogroup O22. The neutral polymer has a linear structure with the repeating unit shown. The same tetrasaccharide unit also forms the backbone of the branched neutral polymer isolated from the reference strain for serogroup O10, which cross-reacts strongly with O22. ----2)-alpha-L-Rhap-(1----2)-alpha-L-Rhap-(1----3)-alpha-L-+ ++Rhap-(1----3)-alpha- D-GlcpNAc-(1----

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Carbohydrate research, 203, 0008-6215, 247-51, 1990

PMID: 2276124

A common structure for neutral polymers isolated from the lipopolysaccharides of reference strains for Serratia marcescens serogroups O17 and O19.
Oxley D,Wilkinson SG

Carbohydrate research, 198, 0008-6215, 168-72, 1990

PMID: 2191776

Structural studies of acidic polymers produced by the O23 reference strain of Serratia marcescens: presence of amide-linked glutamic acid.
Oxley D,Wilkinson SG

The major fraction of an acidic galactoglucomannan present in lipopolysaccharide extracts from cell walls of the O23 reference strain of Serratia marcescens has the tetrasaccharide repeating-unit shown. In a minor fraction, L-glutamic acid was amide-linked to about half of the D-glucuronic acid residues. The possible contributions of the acidic polymers and a neutral polymer produced by the organism to cross-reactions with other serogroups are discussed.

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Carbohydrate research, 204, 0008-6215, 85-91, 1990

PMID: 1980630

Structure of a mannan isolated from the lipopolysaccharide of the reference strain (S3255) for a new serogroup of Serratia marcescens.
Oxley D,Wilkinson SG

Both a neutral and an acidic polymer have been isolated from a lipopolysaccharide extract of Serratia marcescens strain S3255. The neutral polymer is a linear mannan with the repeating unit shown. The same repeating unit has been described for the O-specific polymers from Escherichia coli O8 and Klebsiella O5.

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Carbohydrate research, 212, 0008-6215, 213-7, 1991

PMID: 1959117

Structure of an acidic glycan present in the lipopolysaccharide extract from the reference strain for Serratia marcescens serogroup O18.
Oxley D,Wilkinson SG

The lipopolysaccharide extract from the cell wall of the reference strain for Serratia marcescens serogroup O18 contained, in addition to a neutral glycan characterised previously, an acidic glycan. Acidity was contributed both by D-glucuronic acid and by 4-O-[(R)-1-carboxyethyl]-D-glucose (4-O-Lac-D-Glc). By using n.m.r. spectroscopy, methylation analysis, and chemical degradations, the repeating unit of the acidic glycan was identified as a branched hexasaccharide having the structure shown; an O-acetyl group also present was not located. The glycan is believed to define the O18 serogroup, but is probably not an integral component of the lipopolysaccharide. [formula: see text].

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Carbohydrate research, 215, 0008-6215, 293-301, 1991

PMID: 1794127

Structure of the 021 antigen from Serratia marcescens.
Oxley D,Wilkinson SG

Lipopolysaccharide was isolated from both phases of an aqueous-phenol extraction of defatted cell walls from the reference strain for Serratia marcescens serogroup 021. The product from the aqueous phase was of the R type, lacking a polymeric side-chain. The polymeric fraction of the lipopolysaccharide from the phenolic phase (the 021 antigen) had a disaccharide repeating-unit with the following structure: ----4)-alpha-D-Glcp-(1----4)-beta-D-ManpNAc-(1----.

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Carbohydrate research, 212, 0008-6215, 187-92, 1991

PMID: 1720344

Structure of a glucorhamnan from the lipopolysaccharide of Serratia marcescens strain S1254.
Oxley D,Wilkinson SG

Carbohydrate research, 209, 0008-6215, 319-22, 1991

PMID: 1709820

Structure of the putative O23 antigen of Serratia marcescens.
Oxley D,Wilkinson SG

A neutral glycan containing L-rhamnose and 2-acetamido-2-deoxy-D-galactose is one of two polymers present in the lipopolysaccharide extract from the reference strain for Serratia marcescens serogroup O23. The glycan, which has the disaccharide repeating-unit shown, shares structural features with polymers from several other O serogroups. ----4)-alpha-L-Rhap-(1----4)-beta-D-GalpNAc-(1----.

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Carbohydrate research, 196, 0008-6215, 127-31, 1990

PMID: 1693310

Structure of an acidic glycan from the reference strain for Serratia marcescens serogroup O22.
Oxley D,Wilkinson SG

In addition to a neutral glycan, lipopolysaccharide extracts from the reference strain for Serratia marcescens serogroup O22 contain an acidic polymer which probably defines the serogroup and is of microcapsular origin. The polymer is doubly branched with a heptasaccharide repeating unit and a galactan backbone. By means of spectroscopic and degradative studies, the structure of the repeating unit was established as that shown. [formula: see text]

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Carbohydrate research, 231, 0008-6215, 237-48, 1992

PMID: 1394317

Structure of a neutral glycan from the lipopolysaccharides of reference strains for Serratia marcescens serogroups O2 and O3.
Oxley D,Wilkinson SG

Serogroups O2 and O3 of Serratia marcescens are differentiated by acidic glycans present in the aqueous phase when lipopolysaccharides are extracted from the reference strains by the aqueous-phenol method. The phenolic phases of these extracts from both strains also contain lipopolysaccharides, from which the same neutral glycan is released on milk acid hydrolysis. The neutral glycan has the disaccharide repeating-unit shown, and accounts for the cross-reactions between the two serogroups and also with serogroup O21: --> 4)-alpha-D-GlcpNAc-(1-->4)-beta-D-ManpNAc-(1--.

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FEMS microbiology letters, 78, 0378-1097, 209-12, 1992

PMID: 1283379