Liquid chromatography or gas chromatography hyphenated to triple quadrupole mass spectrometers for targeted lipidomics:
AB SCIEX 6500 QTRAP coupled with Shimadzu Prominence HPLC is used for multiple reaction monitoring (MRM) assays. This system is more sensitive than the orbitrap Elite and Velos Pro allowing semi and absolute quantitation of lipid subclasses in the range of pg in sample.
The SCIEX 6500 can be coupled with a differential ion mobility SelexION device. Mobility separation provides an extra level of selectivity between the chromatography and the mass spectrometer for a further degree of separation of coeluting compounds, isomers, and low abundant compounds buried in the background noise.