Life Sciences Research for Lifelong Health
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Wide-field Imaging

Wide-field fluorescence is the most straightforward method of fluorescence microscopy.  Typically, excitation light is directed through the objective lens (episcopic illumination) with the same lens used to capture the emitted fluorescence light which is then detected by eye or with a camera.  The non-selective illumination and non-selective detection mean that this method is not appropriate for thicker specimens where out-of-focus light can degrade the image.  It is, however, very useful for routine work with thin specimens and low light imaging applications such as live cell imaging.

The Imaging Facility has several wide-field fluorescence microcopes, all of which are configured for live cell imaging.

Our Systems:

Nikon Ti-E

Full enclosure incubation plus CO2
4x, 10x, 20x, 40x, 60x & 100x objectives
Perfect focus system
DIC optics
sCMOS camera
LED illuminator
Fast filter wheel

Olympus cellSens

Full enclosure incubation with CO2
4x, 10x, 20x, 40x, 60x (silicone), 100x objectives
Focus lock
DIC optics
Fully motorised
sCMOS camera
LED illuminator
Fast filter wheels

Olympus CellR

Full enclosure incubation
4x, 10x, 20x, 40x, 60x, 100x objectives
DIC optics
Polychromator light source
CCD camera
Fast filter wheel
Optimised for calcium imaging applications