Head of Flow Cytometry: Dr Rachael Walker
Building 580, Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT
Rachael has over a dozen years of experience in flow cytometry and cell sorting and nearly 10 years of working in flow cytometry core facilities. Rachael joined the core in September 2012, following 7 years running Flow Cytometry Core Facilities at the University of Cambridge.
Rachael has extensive experience in analysis and sorting cells of differing types including; immunology, cell biology, stem cell biology, large cells such as cardiomyocytes, c. elegans
eggs; organelles such as nuclei. Rachael can provide expertise in experimental setup, optimisation and analysis. She can help with optimal instrument set up, post-acquisition analysis of data and preparing figures for papers.
2005- PhD in Tissue Engineering, Department of Clinical Engineering, University of Liverpool
2001- BMedSc (Honours), Biomedical Materials Science, University of Birmingham
Flow Cytometry community
Rachael is very involved with the flow cytometry community on a local, national and international level.
Rachael’s work includes:
- Awarded International Society for Advancement of Cytometry (ISAC) Scholarship 2012-2014
- Awarded ISAC ‘Emerging Leader in Shared Resource Laboratory’ Scholarship 2014-2016
- Co-chair of ISAC Membership Services Committee
- Member of ISAC Program committee for CYTO conferences since 2010
- Secretary and organiser of local Mid-Anglia Cytometry (MACC) meetings
- Secretary of flowcytometryUK, also one of main organisers of flowcytometryUK national annual meetings
- Fellow and Secretary of Royal Microscopical Society (RMS) Cytometry Section
- Lecturer at RMS Annual Flow Cytometry Course
- Regular contributor to ‘Flow Cytometry’ Channel on Bitesize Bio website.
Tracking the embryonic stem cell transition from ground state pluripotency.
Kalkan T, Olova N, Roode M, Mulas C, Lee HJ, Nett I, Marks H, Walker R, Stunnenberg HG, Lilley KS, Nichols J, Reik W, Bertone P, Smith A
Mouse embryonic stem (ES) cells are locked into self-renewal by shielding from inductive cues. Release from this ground state in minimal conditions offers a system for delineating developmental progression from naive pluripotency. Here we examined the initial transition process. The ES cell population behaves asynchronously. We therefore exploited a short-half-life Rex1::GFP reporter to isolate cells either side of exit from naive status. Extinction of ES cell identity in single cells is acute. It occurs only after near-complete elimination of naïve pluripotency factors, but precedes appearance of lineage specification markers. Cells newly departed from the ES cell state display features of early post-implantation epiblast and are distinct from primed epiblast. They also exhibit a genome-wide increase in DNA methylation, intermediate between early and late epiblast. These findings are consistent with the proposition that naive cells transition to a distinct formative phase of pluripotency preparatory to lineage priming.
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Development (Cambridge, England), , 1477-9129, , 2017
International Society for Advancement of Cytometry (ISAC) flow cytometry shared resource laboratory (SRL) best practices.
Barsky LW, Black M, Cochran M, Daniel BJ, Davies D, DeLay M, Gardner R, Gregory M, Kunkel D, Lannigan J, Marvin J, Salomon R, Torres C, Walker R
The purpose of this document is to define minimal standards for a flow cytometry shared resource laboratory (SRL) and provide guidance for best practices in several important areas. This effort is driven by the desire of International Society for the Advancement of Cytometry (ISAC) members in SRLs to define and maintain standards of excellence in flow cytometry, and act as a repository for key elements of this information (e.g. example SOPs/training material, etc.). These best practices are not intended to define specifically how to implement these recommendations, but rather to establish minimal goals for an SRL to address in order to achieve excellence. It is hoped that once these best practices are established and implemented they will serve as a template from which similar practices can be defined for other types of SRLs. Identification of the need for best practices first occurred through discussions at the CYTO 2013 SRL Forum, with the most important areas for which best practices should be defined identified through several surveys and SRL track workshops as part of CYTO 2014. © 2016 International Society for Advancement of Cytometry.
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Cytometry. Part A : the journal of the International Society for Analytical Cytology, 89, 1552-4930, 1017-1030, 2016
The spindle assembly checkpoint works like a rheostat rather than a toggle switch.
Collin P,Nashchekina O,Walker R,Pines J
The spindle assembly checkpoint (SAC) is essential in mammalian mitosis to ensure the equal segregation of sister chromatids. The SAC generates a mitotic checkpoint complex (MCC) to prevent the anaphase-promoting complex/cyclosome (APC/C) from targeting key mitotic regulators for destruction until all of the chromosomes have attached to the mitotic apparatus. A single unattached kinetochore can delay anaphase for several hours, but how it is able to block the APC/C throughout the cell is not understood. Present concepts of the SAC posit that either it exhibits an all-or-nothing response or there is a minimum threshold sufficient to block the APC/C (ref. 7). Here, we have used gene targeting to measure SAC activity, and find that it does not have an all-or-nothing response. Instead, the strength of the SAC depends on the amount of MAD2 recruited to kinetochores and on the amount of MCC formed. Furthermore, we show that different drugs activate the SAC to different extents, which may be relevant to their efficacy in chemotherapy.
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Nature cell biology, 15, 1476-4679, 1378-85, 2013