Flow Facility Equipment
AnalysersBecton Dickinson LSR Fortessa 4
Becton Dickinson LSR Fortessa 5
Becton Dickinson LSRII
Becton Dickinson FACSCalibur
Cell SortersBecton Dickinson FACSAriaIII
Becton Dickinson Influx
Other EquipmentMerck Millipore Amnis Imagestream MkII
Merck Millipore Muse Cell counter
We also hold a FlowJo Site license for Babraham Research Campus
Becton Dickinson LSR Fortessa 44 lasers, 15 parameters (488nm, 561nm, 405nm and 640nm lasers)
This analyser was added to the core in early 2012 and is a heavily used vital piece of equipment for the research that is carried out at Babraham Institute. This machine allows 13 colours to be simultaneously measured as well as 2 scatter parameters. This machine is equipped with a 561nm laser, which allows detection of red fluorescent proteins and better excitation of PE fluorochromes. Utilising this laser line also reduces spillover and compensation with other channels such as FITC. Since purchase this machine has proved very popular and is our most regularly used analyser.
Becton Dickinson LSR Fortessa 55 lasers, 20 parameters (488nm, 561nm, 405nm, 355nm and 640nm lasers)
This state of the art cytometer is our latest addition to the core facility, purchased in April 2014, due to the popularity of our original Fortessa.
With the increasing trend for polychromatic flow cytometry, this sophisticated equipment permits use of up to 18 fluorochromes simultaneously plus 2 scatter parameters. This machine is proving an invaluable asset as it allows scientists to maximise data acquired from each sample, saving time, money and valuable cells/tissues. This machine has also been equipped with a UV laser, allowing scientists to use the new BD Brilliant UV dyes.
The new Fortessa is also equipped with a High-throughput sampler, which can run 96 and 384 well plates. This means that a 96 well plate can be read in under an hour.
Becton Dickinson LSRII4 laser, 13 parameters (488nm, 405nm. 355nm and 640nm lasers)
The LSRII has been part of the core facility since 2006 and is a much loved machine. Equipped with a UV laser, it allows scientists to look at Calcium Flux, DAPI/Hoechst cell cycle as well as UV excited fluorochromes.
Becton Dickinson FACSCalibur2 lasers, 6 parameters (488nm and 635nm lasers)
Purchased in 1996, this long-standing member of the core, is a stable machine for simple experiments requiring only a couple of colours. It is ideal for PI cell cycle analysis or just simple GFP analysis.
Becton Dickinson FACSAria III4 lasers, 13 parameters (488nm, 561nm, 405nm and 640nm lasers)
Our Aria sorter is the workhorse of the facility and is sorting for up to 7 hours a day. This sorter has been upgraded to include a 561nm laser, allowing for the excitation of RFPs and better excitation of PE conjugates. The layout of this machine mirrors the Fortessa 4, meaning that experiments can easily be transferred from analyser to sorter.
Designed with flexibility in mind, differing nozzle sizes and pressures are used depending on cell types/applications. The nozzles that are available are 70μm, 85μm, 100μm and 130μm.
The Aria can sort up to 4 populations simultaneously, sorting into tubes ranging from eppendorfs to 50 ml Falcons and can also sort into plates. The sorting into 96 well plates allows single cell cloning of cell populations to be achieved in a matter of minutes.
Becton Dickinson Influx High-Speed Cell Sorter5 laser, 13 parameters (488nm, 561nm, 405nm, 355nm and 640nm lasers)
An addition to the core in 2011, this speedy sorter is housed in a Containment Level 2 hood. The Influx has recently been upgraded to 6 way sorting and an additional UV laser was added to the family of 4 existing lasers. The UV laser is already been successfully used for sorting phases of the cell cycle using Hoechst dye and is ready for the new Becton Dickinson Brilliant UV dyes.
The Influx sorter can sort into Eppendorfs, 5ml tubes, 15ml and 50 ml Falcons. It also has a plate-sorting arm for single cell cloning (or in fact any number of cells into any wells of any standard sized tissue culture plates).
Within the Facility, utilising the Influx Class II hood, it is possible to sort non-tested human samples (subject to risk assessments being reviewed by the Institute Biosafety committee).
Merck Millipore Amnis Imagestream4 laser, 12 parameter (488nm, 561nm, 405nm and 640nm lasers)
The Imagestream is an image cytometer that allows quantitative data to be acquired as well as images of every event passing through the instrument to be recorded. This machine can analyse up to 5000 cells/sec. This clever machine allows the user to look at features of cells such as co-localisation, staining location, cell-cell location, spot counting and cell death analysis.
Members of the core facility are available to help set up and optimise assays and for help with post-acquisition analysis of the data. Babraham Institute is currently the only Academic Institute in Cambridge to have this exciting technology.
Merck Millipore Muse Cell Analyser1 laser, 3 Parameters (532nm laser)
This mini cytometer, allows assessment of cell viability, apoptosis and cell cycle. The machine is simple to use and delivers results quickly and accurately.