Life Sciences Research for Lifelong Health

Flow Facility Equipment

All equipment is available for external use, please contact flow@babraham.ac.uk for more information about core facility and using the facility.  All of our analysers and most of our sorters have a 355nm laser to allow scientists to utilise fluorochromes from a UV laser.

Equipment List

Analysers

Becton Dickinson LSR Fortessa 4
Becton Dickinson LSR Fortessa 5
Becton Dickinson LSRII
Becton Dickinson FACSCalibur

Cell Sorters

Becton Dickinson FACSAriaIII
Becton Dickinson Influx

Other Equipment

Merck Millipore Amnis Imagestream MkII
Merck Millipore Muse Cell counter
We also hold a FlowJo Site license for Babraham Research Campus



Analysers

​Becton Dickinson LSR Fortessa 4

4 lasers, 15 parameters (488nm, 561nm, 405nm and 640nm lasers)
This analyser was added to the core in early 2012 and is a heavily used vital piece of equipment for the research that is carried out at Babraham Institute.  This machine allows 13 colours to be simultaneously measured as well as 2 scatter parameters.  This machine is equipped with a 561nm laser, which allows detection of red fluorescent proteins and better excitation of PE fluorochromes.  Utilising this laser line also reduces spillover and compensation with other channels such as FITC.  Since purchase this machine has proved very popular and is our most regularly used analyser.
Fortessa logo

​Becton Dickinson LSR Fortessa 5

5 lasers, 20 parameters (488nm, 561nm, 405nm, 355nm and 640nm lasers)
 
This state of the art cytometer is our latest addition to the core facility, purchased in April 2014, due to the popularity of our original Fortessa. 
With the increasing trend for polychromatic flow cytometry, this sophisticated equipment permits use of up to 18 fluorochromes simultaneously plus 2 scatter parameters.  This machine is proving an invaluable asset as it allows scientists to maximise data acquired from each sample, saving time, money and valuable cells/tissues.  This machine has also been equipped with a UV laser, allowing scientists to use the new BD Brilliant UV dyes.

The new Fortessa is also equipped with a High-throughput sampler, which can run 96 and 384 well plates. This means that a 96 well plate can be read in under an hour. 
Fortessa machine

​Becton Dickinson LSRII

4 laser, 13 parameters (488nm, 405nm. 355nm and 640nm lasers)
 
The LSRII has been part of the core facility since 2006 and is a much loved machine.  Equipped with a UV laser, it allows scientists to look at Calcium Flux, DAPI/Hoechst cell cycle as well as UV excited fluorochromes. 
Flow engineers names

​Becton Dickinson FACSCalibur

2 lasers, 6 parameters (488nm and 635nm lasers)
 
Purchased in 1996, this long-standing member of the core, is a stable machine for simple experiments requiring only a couple of colours.  It is ideal for PI cell cycle analysis or just simple GFP analysis. 
FACS Calibur machine



Cell Sorters

Becton Dickinson FACSAria III

4 lasers, 13 parameters (488nm, 561nm, 405nm and 640nm lasers)
 
Our Aria sorter is the workhorse of the facility and is sorting for up to 7 hours a day.  This sorter has been upgraded to include a 561nm laser, allowing for the excitation of RFPs and better excitation of PE conjugates.  The layout of this machine mirrors the Fortessa 4, meaning that experiments can easily be transferred from analyser to sorter. 
 
Designed with flexibility in mind, differing nozzle sizes and pressures are used depending on cell types/applications.  The nozzles that are available are 70μm, 85μm, 100μm and 130μm.
 
The Aria can sort up to 4 populations simultaneously, sorting into tubes ranging from eppendorfs to 50 ml Falcons and can also sort into plates.  The sorting into 96 well plates allows single cell cloning of cell populations to be achieved in a matter of minutes.
Flow cytometer closeup angle left

Becton Dickinson Influx High-Speed Cell Sorter

5 laser, 13 parameters (488nm, 561nm, 405nm, 355nm and 640nm lasers)
 
An addition to the core in 2011, this speedy sorter is housed in a Containment Level 2 hood.  The Influx has recently been upgraded to 6 way sorting and an additional UV laser was added to the family of 4 existing lasers.  The UV laser is already been successfully used for sorting phases of the cell cycle using Hoechst dye and is ready for the new Becton Dickinson Brilliant UV dyes.
 
The Influx sorter can sort into Eppendorfs, 5ml tubes, 15ml and 50 ml Falcons.  It also has a plate-sorting arm for single cell cloning (or in fact any number of cells into any wells of any standard sized tissue culture plates). 
 
Within the Facility, utilising the Influx Class II hood, it is possible to sort non-tested human samples (subject to risk assessments being reviewed by the Institute Biosafety committee).
 ​
Flow lasers wide

Other Equipment

Merck Millipore Amnis Imagestream

4 laser, 12 parameter (488nm, 561nm, 405nm and 640nm lasers)
 
The Imagestream is an image cytometer that allows quantitative data to be acquired as well as images of every event passing through the instrument to be recorded.  This machine can analyse up to 5000 cells/sec.  This clever machine allows the user to look at features of cells such as co-localisation, staining location, cell-cell location, spot counting and cell death analysis.
Members of the core facility are available to help set up and optimise assays and for help with post-acquisition analysis of the data.  Babraham Institute is currently the only Academic Institute in Cambridge to have this exciting technology.
 
 
Imagestrream machine

Merck Millipore Muse Cell Analyser

1 laser, 3 Parameters (532nm laser)
  
This mini cytometer, allows assessment of cell viability, apoptosis and cell cycle.  The machine is simple to use and delivers results quickly and accurately.
 ​
 
 
Flow lasers wide



All pieces of equipment are available for external use, please contact flow@babraham.ac.uk for more information about core facility and using the facility.  All of our analysers and most of our sorters have a 355nm laser to allow scientists to utilise fluorochromes from a UV laser. 

Sorters

All of the sorters in the BI Flow Cytometry Core Facility are available as part of the cell sorting service run by the facility.

Flow cytometer closeup angle left

BD Aria III

4 lasers (488nm, 405nm, 561nm, 640nm) 16 fluorescent colours, capable of sorting 4 populations simultaneously. This machine can be used for plate sorting and index sorting.
Flow lasers wide

BD Influx

5 lasers (488nm, 405nm, 561nm, 640nm, 355nm, 15 colours. Can separate 6 populations simultaneously. The Influx is in a Class 2 hood which allows sorting of human cell lines and human primary cells/tissues. This machine can be used for plate sorting and Index sorting.
Fusion

BD FACS Aria Fusion

5 lasers (488nm, 405nm, 561nm, 640nm, 355nm, 18 colours. The Fusion is in a Class 2 hood which allows sorting of human cell lines and human primary cells/tissues. This machine can be used for plate sorting and index sorting.

Analysers

3 x BD LSRFortessa. 5 lasers (488nm, 405nm, 561nm, 640nm, 355nm), able to detect 18 colours, including red fluorescent proteins.

Fortessa machine

Fortessa 5a

This state of the art cytometer was purchased in April 2014, due to the popularity of our original Fortessa. With the increasing trend for polychromatic flow cytometry, this sophisticated equipment permits use of up to 18 fluorochromes simultaneously plus 2 scatter parameters.

This machine is proving an invaluable asset as it allows scientists to maximise data acquired from each sample, saving time, money and valuable cells/tissues. This machine has also been equipped with a UV laser, allowing scientists to use the new BD Brilliant UV dyes.

This Fortessa is also equipped with a High-throughput sampler, which can run 96 and 384 well plates.
Fortessa

Fortessa 5b

This analyser was added to the core in early 2012 (and upgraded to 5 lasers in 2016) and is a heavily used vital piece of equipment for the research that is carried out at Babraham Institute.

This machine allows 18 colours to be simultaneously measured as well as 2 scatter parameters. This machine is equipped with a 561nm laser, which allows detection of red fluorescent proteins and better excitation of PE fluorochromes. Utilising this laser line also reduces spillover and compensation with other channels such as FITC.

Since purchase this machine has proved very popular and is our most regularly used analyser.
Flow oscilloscope

Fortessa 5c

Our latest addition to the Flow Core was purchased in March 2016 to complement the exisiting Fortessa analysers.

As well as having 5 lasers for 18 colour analysis this instrument was configured to maximize the use of BD Brilliant UV dyes. It has been set up to allow 3 parameters to be analysed from the UV laser).

The new Fortessa is also equipped with a High-throughput sampler, which can run 96 and 384 well plates.

Other equipment

Amnis Imagestream

4 laser (488nm, 405nm, 561nm, 640nm). This imaging cytometer produces multiple high resolution images of every cell directly in flow, giving quantitative information about each cell in up to 12 channels simultaneously. The Imagestream is an image cytometer that allows quantitative data to be acquired as well as images of every event passing through the instrument to be recorded. This machine can analyse up to 5000 cells/sec. This clever machine allows the user to look at features of cells such as co-localisation, staining location, cell-cell location, spot counting and cell death analysis. Members of the core facility are available to help set up and optimise assays and help with post-acquisition analysis of the data.
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Merck Millipore Muse

1 laser, 3 Parameters (532nm laser) This mini cytometer allows assessment of cell viability, apoptosis and cell cycle. The machine is simple to use and delivers results quickly and accurately. This machine is regualry used for cell counting.

Miltenyi Biotec Automacs

This automated benchtop instrument uses Miltenyi MACS Microbead technology to bulk separate multiple samples.