Life Sciences Research for Lifelong Health

Tim Pearce

The Biological Support Unit is managed by Tim Pearce. Tim joined the Institute in 1979 as a junior animal technician. He had a short break of five years returning in 1995 in the role of containment unit Supervisor and then Manager. Tim gained significant knowledge of various disciplines and went on to become general manager of the centralised BI facility in 2000. He was offered and took the full time role of client consultant for the proposed new BSU construction project in 2006 following the project through to completion in Sept 2009. The new BSU facility is highly regarded in the industry and has received recognition and visitors from around the globe. Tim became Head of Facility in 2010, facilitating the migration and set up of the new unit.  During 2012 Tim joined a project to extend the new BSU facility which was completed in July 2014. He is a member of the Institute of Animal technicians and the Laboratory Animal Sciences Association and represents the facility in various senior committees on site.  

Latest Publications

Role of the p110delta PI 3-kinase in integrin and ITAM receptor signalling in platelets.
Senis YA, Atkinson BT, Pearce AC, Wonerow P, Auger JM, Okkenhaug K, Pearce W, Vigorito E, Vanhaesebroeck B, Turner M, Watson SP

We have investigated the function of the p110delta catalytic subunit of phosphoinositide 3-kinase (PI 3-kinase) in platelets using p110delta knock-out (p110delta(-/-)) mice and p110delta knock-in (p110delta(D910A/D910A)) mice, which express a catalytically inactive form of the enzyme. Aggregation to threshold concentrations of the GPVI-specific agonist, CRP, was partially reduced in p110delta(-/-) and p110delta(D910A/D910A) platelets. This inhibition was overcome by higher concentrations of CRP. The degree of inhibition was considerably weaker than that induced by LY294002 and wortmannin, which inhibit all isoforms of PI 3-kinase. p110delta(-/-) platelets showed decreased spreading on fibrinogen- or von Willebrand factor (VWF)-coated surfaces under static conditions, whereas they spread normally on collagen. LY294002 had a more pronounced inhibitory effect on spreading on all three surfaces. Adhesion and aggregate formation of p110delta(-/-) platelets to collagen or fibrinogen/VWF at intermediate/high rates of shear were normal. This study demonstrates a minor role for the p110delta catalytic subunit in mediating platelet activation by the collagen receptor GPVI and integrin alphaIIbeta3. The more pronounced inhibitory effect of LY294002 and wortmannin indicates that other isoforms of PI 3-kinase play a more significant role in signalling by the two platelet glycoprotein receptors.

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Platelets, 16, 0953-7104, 191-202, 0

PMID: 16011964

 

Latest Publications

Role of the p110delta PI 3-kinase in integrin and ITAM receptor signalling in platelets.

Senis YA, Atkinson BT, Pearce AC

Platelets
16 0953-7104:191-202 (0)

PMID: 16011964

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