Life Sciences Research for Lifelong Health

Protocols

Single-cell Hi-C

Visit Nature Protocols site with the following link:
Single-cell Hi-C for genome-wide detection of chromatin interactions that occur simultaneously in a single cell
(2015) Takashi Nagano, Yaniv Lubling, Eitan Yaffe, Steven W Wingett, Wendy Dean, Amos Tanay & Peter Fraser
Nature protocols 10, 1986–2003.

Original research publication using this method:
Single-cell Hi-C reveals cell-to-cell variability in chromosome structure (2013) T Nagano, Y Lubling, TJ Stevens, S Schoenfelder, E Yaffe, W Dean, ED Laue, A Tanay & P Fraser
Nature 502 (7469), 59-64.
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Enhanced 4C

Click on link below to download a copy of the protocol:
Sensitive detection of chromatin co-associations using enhanced chromosome conformation capture on chip

or visit Nature Protocols site with the following link:
Sensitive detection of chromatin coassociations using enhanced chromosome conformation capture on chip
(2012) T Sexton, S Kurukuti, JA Mitchell, D Umlauf, T Nagano, P Fraser
Nature protocols 7 (7), 1335-1350.

Original research publication using this method:
Preferential associations between co-regulated genes reveal a transcriptional interactome in erythroid cells
(2010) Stefan Schoenfelder, Tom Sexton, Lyubomira Chakalova, Nathan F Cope, Alice Horton, Simon Andrews, Sreenivasulu Kurukuti, Jennifer A Mitchell, David Umlauf, Daniela S Dimitrova, Christopher H Eskiw, Yanquan Luo, Chia-Lin Wei, Yijun Ruan, James J Bieker & Peter Fraser
der, T Sexton, L Chakalova, NF Cope, A Horton, S Andrews, ...
Nature genetics 42 (1), 53-61