The last ~ 15 years have shown that histone modifications, histone variants, nucleosome remodeling, nonhistone proteins, chromatin fiber looping and nuclear organization represent different levels of a coherent regulatory network.
A critical challenge of molecular biology is to understand how this incredibly complex packaging of the genome is involved in regulating its function. We study how the organization of the genome impacts key activities in the nucleus and how changes of this organization are achieved and controlled at the molecular level.
In particular, we explore how DNA is packaged into specific types of chromatin during and following DNA replication and what happens, if this process is perturbed. We identify factors that are involved in these processes and explore their mechanisms of action.
Our work identified ATP-dependent chromatin remodeling factors as key factors of the chromatin replication process. We study how these enzymes are involved to ensure epigenomic and genomic stability and examine their role in healthy ageing.