Life Sciences Research for Lifelong Health

Anne Corcoran

Research Summary

The focus of our research is understanding the role of chromatin and nuclear organization in controlling gene expression during the development of the immune system.​

​B lymphocytes are cells of the immune system that produce antibodies (immunoglobulins), which recognise and inactivate foreign antigens like bacteria. To cope with the enormous numbers of foreign antigens encountered during our lifespan, these cells must produce millions of different antibodies.

VDJ recombinationRecombination or ‘shuffling' of genes in the immunoglobulin heavy chain (IgH) locus is the first step in generating this huge repertoire.

Special ‘marks’ on the chromatin are thought to underlie the complex choice of gene segments in the multigenic immunoglobulin gene families, that can be recombined during B cell development to produce a large diversity of functional antibody molecules.

Our group studies non-coding RNA transcription (ie generation of transcripts that do not produce protein) in specific parts of the immunoglobulin cluster, which may play a directive role in V(D)J recombination, or mark epigenetic control regions.

Only one of each gene type is used in an individual cell and the resulting DNA sequence encodes a unique IgH, which is expressed with an Ig light chain as a unique highly specific antibody in each cell.

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Latest Publications

Two Mutually Exclusive Local Chromatin States Drive Efficient V(D)J Recombination.

Bolland DJ, Koohy H, Wood AL

Cell reports
15 2211-1247:2475-87 (2016)

PMID: 27264181

RNA-binding proteins ZFP36L1 and ZFP36L2 promote cell quiescence.

Galloway A, Saveliev A, Łukasiak S

Science (New York, N.Y.)
352 1095-9203:453-9 (2016)

PMID: 27102483

Robust 3D DNA FISH using directly labeled probes.

DJ Bolland, MR King, W Reik

Journal of visualized experiments : JoVE
78: (2013)

DOI: 10.3791/50587

PMID: 23978815

Non-coding transcription and large-scale nuclear organisation of immunoglobulin recombination.

MJ Stubbington, AE Corcoran

Current opinion in genetics & development
23 2:81-8 (2013)

DOI: 10.1016/j.gde.2013.01.001

PMID: 23434028

Highly restricted usage of Ig H chain VH14 family gene segments in Slp65-deficient pre-B cell leukemia in mice.

VB Ta, MJ de Bruijn, L Matheson

Journal of immunology (Baltimore, Md. : 1950)
189 10:4842-51 (2012)

DOI: 10.4049/jimmunol.1201440

PMID: 23066158

Editorial overview. Lymphocyte development.

AE Corcoran, AJ Feeney

Current opinion in immunology
24 2:129-31 (2012)

DOI: 10.1016/j.coi.2012.03.003

PMID: 22440337

Local and global epigenetic regulation of V(D)J recombination.

LS Matheson, AE Corcoran

Current topics in microbiology and immunology
356 :65-89 (2012)

DOI: 10.1007/82_2011_137

PMID: 21695632